Preliminary steps
👉 This tutorial requires that you have installed node template Import images
.
If you do not have these templates, you can download them via Manage > Download more templates
or by importing the Templates.json
file that is provided in the data package. If you do not know how to download or import node templates, please check out our tutorial.
Step 1
Create a new JIPipe project (red arrow 1) using a template (red arrow 2).
Step 2
Create a new JIPipe project (red arrow 1) using a template (red arrow 2).
Step 3
Locate the data
folder that belongs to this tutorial (red arrow 1) and drop it on the UI (red arrow 2).
Click somewhere on the white area of the UI (red arrow 3) and chose the Node templates
tab (red arrow 4).
Select the pre-made Import images
template from the list (red arrow 5) and drag it to the UI. Connect it to the Folder list
node (red arrow 6) and run the Import images
node (red arrow 7.)
Step 4
The Cache browser
(red arrow 1) will now show the fluorescence image (red arrow 2).
Step 5
Add the Basic blob finder
template to the UI (red arrow 1) and observe the Parameters
tab (red arrow 2).
The exposed parameter of the group node are indicated here (red rectangle 3), including the particle size and circularity ranges, the thresholding method and the gaussian smoothing factor.
Step 6
In order to observe the quality of the segmentation, add a Convert ROI to RGB
node (red arrow 1), connect it to the ROI
and Image
outputs of the Import images
and Basic blob finder
nodes (red arrows 2 and 3), and run it (red arrow 4).
In the Cache browser
(red arrow 5), observe the entry (red arrow 6) and the full image (red arrow 7).
Step 7
Look for Table processing nodes in the Tables
menu (red arrow 1) and select the node Apply expression per row
(red arrow 2).
Connect the node to the Measurements
output of the Simple blob finder
.
Step 8
Before editing the table, find out the names of the measured parameters:
open the Measurements cache results of the Basic blob finder
(red arrow 1), double-click on the cache entry (rectangle 2) and review the column names in the table viewer (rectangle 3).
Step 9
Let us calculate the ratio between the mean value and the area.
Select the Apply expression per row
node (red arrow 1), open the Parameters
tab and apply the following changes:
In parameter Expressions
set the value of Expression
to Mean / Area
.
In parameter Expressions
set the value of Column name
to Density
.
Step 10
Run the node (red arrow 1) and observe the Cache browser
(red arrow 2).
The cache entry (red arrow 3) now contains a new Density
column (red arrow 4).
Step 11
We will proceed to generate an integrated table.
From the Tables
menu (red arrow 1), add the node Integrate table columns
(red arrow 2).
Step 12
Select the Integrate table columns
(red arrow 1) and edit the Processors
parameters in the Parameters
tab (red arrow 2):
Set the Input column
to the following value:
"Density"
Proceed by choosing the Average
as a mode of operation (red arrow 4), and provide a name for the new results (e.g., AVG density
, red arrow 5).
Step 13
Run the node (red arrow 1) and observe the Cache (red arrow 2).
The new cache entry (red arrow 3) now contains the new column AVG density
(red arrow 4, red rectangle 5).
Step 14
Finally, add three input nodes to the compartment’s output node and connect them accordingly. (red arrows 1-3).