Required hardware and software
We will provide you with a seat that comes with a PC, screen, mouse, and keyboard. If you want to use your own hardware (laptop), please follow our recommendations:
Operating system: Windows 10/11 or Linux (macOS is technically supported, but we cannot 100% promise that it will work perfectly)
System memory (RAM): At least 16GB
Please download the correct JIPipe version: please go with the latest JIPipe 6.0.0 snapshot that you can find here: https://jipipe.hki-jena.de/download-snapshot
We will need features that are only present in JIPipe 6.
A part of the course will be the download and installation of JIPipe.
Important: We recommend you do not install JIPipe as an ImageJ plugin into your existing Fiji - JIPipe requires a special installation procedure if you install it as plugin. Please go with the prepackaged standalone installations that are prominently featured on the homepage.
Interactive workshop data
Provided through the Google Drive
Direct download: https://drive.google.com/file/d/141gzkkit91RjgYY30glrMTHb0mbJEq3v/view?usp=drive_link
Additional data (may or may not be needed): https://drive.google.com/file/d/1dtolrvTHcvVEU3UPUNXNDKByvkNjW1H9/view?usp=sharing
Advanced workshop data
Provided through the Google Drive
Direct download: https://drive.google.com/file/d/1QqWZoRWhGDWrFngi6e-g8EEnlT6_qdut/view?usp=drive_link
Homework
Full package (data+instructions): https://drive.google.com/file/d/1mPXuyHSQV2obA5hLL3u4jRjWr-kLAG8g/view?usp=drive_link
Instructions only (will be also provided as printed copy): https://drive.google.com/file/d/1XTdD05vObXEpzuHopO15rVLmCu-b4U-T/view?usp=drive_link
Folder structure: https://drive.google.com/file/d/1LeHCtzGr0278qM1chMv2bmMdPw4jhmAM/view?usp=drive_link
Additional information (covers cases missed by the instructions): https://drive.google.com/file/d/1EHu5TYxiLbHTCdfMNR-muXWKP6n_xrmL/view?usp=drive_link
We highly encourage you to work together with others to be able to work efficiently and learn from each other!
Also do not underestimate the Microscopy homework!
If you have questions, we will gladly help you!
Additional infos
The batch-processing datasets were organized so there are no blank input images and all inputs have 5 channels
The compartment specification is a suggestion of what nodes may be within each compartment, based on the published workflow. Exact file names, colors, etc. are up to you - just ensure that the tasks are fulfilled
Microscopy question #7 is a bonus question
In question 1.5 we meant to say "cell membrane", NOT nucleus.
Do not forget to check out the "Additional information" PDF file that clarifies some patterns
Do not forget that there's a translation table from ImageJ to JIPipe in the document
Please note that the marker-based segmentation needs segmented nuclei as marker and in JIPipe needs a connected components labeling
New: Since 26.03.2026 : An updated JIPipe 6.0.0 snapshot version was uploaded which allows to simplify the creation of exporter paths. You can access the feature through the new "Build" button
Image analysis task 2: The inputs_full, inputs_reduced, inputs_minimal folders should be the starting point (input folder). No elaborate filtering except for TIFF files is needed.
Image analysis task 3 is a pure organization/project organization task - you do not need additional statistics and operations - just follow what the tasks table says. The specifications are based on the real-world workflow and are only a hint on what actually can be done in addition to the parts you built up in Task 1 and Task 2. If you choose to do the additional visualizations and outputs in Task 3, you'll receive up to 2 bonus points.
Course concluded
Thank you for participating in our course!
All material will be soon published as tutorials.